Biocontrol Activities of Gamma Induced Mutants of Trichoderma harzianum against some Soilborne Fungal Pathogens and their DNA Fingerprinting

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Biocontrol Activities of Gamma Induced Mutants of Trichoderma harzianum against some Soilborne Fungal Pathogens and their DNA Fingerprinting

BACKGROUND Random induced mutation by gamma radiation is one of the genetic manipulation strategies to improve the antagonistic ability of biocontrol agents. OBJECTIVES This study aimed to induce mutants with more sporulation, colonization rate leading to enhanced antagonistic ability (in vitro assay) comparing to wild type (WT) and the assessment of genetic differences (in situ evaluation) u...

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Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens.

Monoconidial cultures of 15 isolates of Trichoderma harzianum were characterized on the basis of 82 morphological, physiological, and biochemical features and 99 isoenzyme bands from seven enzyme systems. The results were subjected to numerical analysis which revealed four distinct groups. Representative sequences of the internal transcribed spacer 1 (ITS 1)-ITS 2 region in the ribosomal DNA ge...

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Trichoderma harzianum : A Biocontrol Agent Effective Against

ELAD, Y., 1. CHET, and J. KATAN. 1980. Trichoderma harzianum: A biocontrol agent effective against Sclerotium rolfsii and Rhizocionia solani. Phytopathology 70:119-121. An isolate of Trichoderma harzianum capable of lysing mycelia of The wheat bran preparation of T. harzianum increased growth of bean Sclerotium rolfsii and Rhizoctonia solani was isolated from a soil naturally plants in a noninf...

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Investigation of Mutation in Chitinase Gene in Gamma Radiated Mutants of Trichoderma Harzianum by STS Marker

Effects of induced mutation via gamma irradiated with 250 Gy dose (in Nuclear Agriculture Research School- Nuclear Science and Technology Research Institute) on Trichoderma harzianum conidiaon chitinase activity by STS molecular marker have been evaluated in this study. Among 20 irradiated mutants which selected via improved antagonistic capability against Rhizoctonia sola...

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ژورنال

عنوان ژورنال: Iranian Journal of Biotechnology

سال: 2016

ISSN: 1728-3043,2322-2921

DOI: 10.15171/ijb.1224